wnt target gene Search Results


rt2 qpcr primer assays for the target genes and housekeeping genes  (Qiagen)
90
Qiagen rt2 qpcr primer assays for the target genes and housekeeping genes
Rt2 Qpcr Primer Assays For The Target Genes And Housekeeping Genes, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rt2 qpcr primer assays for the target genes and housekeeping genes/product/Qiagen
Average 90 stars, based on 1 article reviews
rt2 qpcr primer assays for the target genes and housekeeping genes - by Bioz Stars, 2026-04
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annotated wnt and sox10 transcriptional target gene lists  (GeneGo Inc)
90
GeneGo Inc annotated wnt and sox10 transcriptional target gene lists
a Representative western blots of the indicated proteins in the MAPK inhibitor resistant human melanoma cell culture M12, at different timepoints of CHIR99021 (6 μM) treatment (timepoints are shown directly in the figure). GAPDH was used as loading control ( n = 3). b RNA levels in MAPK inhibitor sensitive (M98) human melanoma cell culture (ΔCt to GAPDH expression) after 4, 8, and 12 h of CHIR99021 (6 µM) treatment are shown compared with DMSO (ctrl) ( n = 3). c Representative western blot of the indicated proteins in the MAPK inhibitor sensitive human melanoma cell culture (M98). A representative western blot for the indicated proteins of M98 human melanoma cell culture stably expressing shSOX10 or shMITF constructs cultured in presence or absence of CHIR99021 for 24 h, 6 μM. Nssh represents the scrambled shRNA used as control construct ( n = 3). d Representative western blot of the indicated proteins in presence of CHIR99021 (6 μM) and/or MG-132 (20 μM) (in single or double treatments) for 16 h. GAPDH was used as a loading control ( n = 3). e Heatmap demonstrating the gene expression <t>of</t> <t>SOX10</t> and <t>Wnt</t> target genes of M98 and M00 (MAPK inhibitor sensitive cell cultures) and M11 (resistant to MAPK inhibitor) in either presence or absence of CHIR99021. Data on the heat map represent log2 of sequencing reads assigned to genes. Statistical significance was determined by unpaired, two-tailed Student’s t test in b . * P < 0.05, ** P < 0.01, *** P < 0.001. In each panel, n indicates the number of independent experiments performed.
Annotated Wnt And Sox10 Transcriptional Target Gene Lists, supplied by GeneGo Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/annotated wnt and sox10 transcriptional target gene lists/product/GeneGo Inc
Average 90 stars, based on 1 article reviews
annotated wnt and sox10 transcriptional target gene lists - by Bioz Stars, 2026-04
90/100 stars
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target gene associated with the wnt signal pathway  (VANGL2 LTD)
90
VANGL2 LTD target gene associated with the wnt signal pathway
a Representative western blots of the indicated proteins in the MAPK inhibitor resistant human melanoma cell culture M12, at different timepoints of CHIR99021 (6 μM) treatment (timepoints are shown directly in the figure). GAPDH was used as loading control ( n = 3). b RNA levels in MAPK inhibitor sensitive (M98) human melanoma cell culture (ΔCt to GAPDH expression) after 4, 8, and 12 h of CHIR99021 (6 µM) treatment are shown compared with DMSO (ctrl) ( n = 3). c Representative western blot of the indicated proteins in the MAPK inhibitor sensitive human melanoma cell culture (M98). A representative western blot for the indicated proteins of M98 human melanoma cell culture stably expressing shSOX10 or shMITF constructs cultured in presence or absence of CHIR99021 for 24 h, 6 μM. Nssh represents the scrambled shRNA used as control construct ( n = 3). d Representative western blot of the indicated proteins in presence of CHIR99021 (6 μM) and/or MG-132 (20 μM) (in single or double treatments) for 16 h. GAPDH was used as a loading control ( n = 3). e Heatmap demonstrating the gene expression <t>of</t> <t>SOX10</t> and <t>Wnt</t> target genes of M98 and M00 (MAPK inhibitor sensitive cell cultures) and M11 (resistant to MAPK inhibitor) in either presence or absence of CHIR99021. Data on the heat map represent log2 of sequencing reads assigned to genes. Statistical significance was determined by unpaired, two-tailed Student’s t test in b . * P < 0.05, ** P < 0.01, *** P < 0.001. In each panel, n indicates the number of independent experiments performed.
Target Gene Associated With The Wnt Signal Pathway, supplied by VANGL2 LTD, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/target gene associated with the wnt signal pathway/product/VANGL2 LTD
Average 90 stars, based on 1 article reviews
target gene associated with the wnt signal pathway - by Bioz Stars, 2026-04
90/100 stars
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wnt-targeted gene therapy systems  (Genlantis inc)
90
Genlantis inc wnt-targeted gene therapy systems
a Representative western blots of the indicated proteins in the MAPK inhibitor resistant human melanoma cell culture M12, at different timepoints of CHIR99021 (6 μM) treatment (timepoints are shown directly in the figure). GAPDH was used as loading control ( n = 3). b RNA levels in MAPK inhibitor sensitive (M98) human melanoma cell culture (ΔCt to GAPDH expression) after 4, 8, and 12 h of CHIR99021 (6 µM) treatment are shown compared with DMSO (ctrl) ( n = 3). c Representative western blot of the indicated proteins in the MAPK inhibitor sensitive human melanoma cell culture (M98). A representative western blot for the indicated proteins of M98 human melanoma cell culture stably expressing shSOX10 or shMITF constructs cultured in presence or absence of CHIR99021 for 24 h, 6 μM. Nssh represents the scrambled shRNA used as control construct ( n = 3). d Representative western blot of the indicated proteins in presence of CHIR99021 (6 μM) and/or MG-132 (20 μM) (in single or double treatments) for 16 h. GAPDH was used as a loading control ( n = 3). e Heatmap demonstrating the gene expression <t>of</t> <t>SOX10</t> and <t>Wnt</t> target genes of M98 and M00 (MAPK inhibitor sensitive cell cultures) and M11 (resistant to MAPK inhibitor) in either presence or absence of CHIR99021. Data on the heat map represent log2 of sequencing reads assigned to genes. Statistical significance was determined by unpaired, two-tailed Student’s t test in b . * P < 0.05, ** P < 0.01, *** P < 0.001. In each panel, n indicates the number of independent experiments performed.
Wnt Targeted Gene Therapy Systems, supplied by Genlantis inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wnt-targeted gene therapy systems/product/Genlantis inc
Average 90 stars, based on 1 article reviews
wnt-targeted gene therapy systems - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
gene sets for wnt pathway components and wnt targets  (Broad Institute Inc)
90
Broad Institute Inc gene sets for wnt pathway components and wnt targets
a Representative western blots of the indicated proteins in the MAPK inhibitor resistant human melanoma cell culture M12, at different timepoints of CHIR99021 (6 μM) treatment (timepoints are shown directly in the figure). GAPDH was used as loading control ( n = 3). b RNA levels in MAPK inhibitor sensitive (M98) human melanoma cell culture (ΔCt to GAPDH expression) after 4, 8, and 12 h of CHIR99021 (6 µM) treatment are shown compared with DMSO (ctrl) ( n = 3). c Representative western blot of the indicated proteins in the MAPK inhibitor sensitive human melanoma cell culture (M98). A representative western blot for the indicated proteins of M98 human melanoma cell culture stably expressing shSOX10 or shMITF constructs cultured in presence or absence of CHIR99021 for 24 h, 6 μM. Nssh represents the scrambled shRNA used as control construct ( n = 3). d Representative western blot of the indicated proteins in presence of CHIR99021 (6 μM) and/or MG-132 (20 μM) (in single or double treatments) for 16 h. GAPDH was used as a loading control ( n = 3). e Heatmap demonstrating the gene expression <t>of</t> <t>SOX10</t> and <t>Wnt</t> target genes of M98 and M00 (MAPK inhibitor sensitive cell cultures) and M11 (resistant to MAPK inhibitor) in either presence or absence of CHIR99021. Data on the heat map represent log2 of sequencing reads assigned to genes. Statistical significance was determined by unpaired, two-tailed Student’s t test in b . * P < 0.05, ** P < 0.01, *** P < 0.001. In each panel, n indicates the number of independent experiments performed.
Gene Sets For Wnt Pathway Components And Wnt Targets, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene sets for wnt pathway components and wnt targets/product/Broad Institute Inc
Average 90 stars, based on 1 article reviews
gene sets for wnt pathway components and wnt targets - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
wnt-targeted gene microarray expression profiling system  (Qiagen)
90
Qiagen wnt-targeted gene microarray expression profiling system
a Representative western blots of the indicated proteins in the MAPK inhibitor resistant human melanoma cell culture M12, at different timepoints of CHIR99021 (6 μM) treatment (timepoints are shown directly in the figure). GAPDH was used as loading control ( n = 3). b RNA levels in MAPK inhibitor sensitive (M98) human melanoma cell culture (ΔCt to GAPDH expression) after 4, 8, and 12 h of CHIR99021 (6 µM) treatment are shown compared with DMSO (ctrl) ( n = 3). c Representative western blot of the indicated proteins in the MAPK inhibitor sensitive human melanoma cell culture (M98). A representative western blot for the indicated proteins of M98 human melanoma cell culture stably expressing shSOX10 or shMITF constructs cultured in presence or absence of CHIR99021 for 24 h, 6 μM. Nssh represents the scrambled shRNA used as control construct ( n = 3). d Representative western blot of the indicated proteins in presence of CHIR99021 (6 μM) and/or MG-132 (20 μM) (in single or double treatments) for 16 h. GAPDH was used as a loading control ( n = 3). e Heatmap demonstrating the gene expression <t>of</t> <t>SOX10</t> and <t>Wnt</t> target genes of M98 and M00 (MAPK inhibitor sensitive cell cultures) and M11 (resistant to MAPK inhibitor) in either presence or absence of CHIR99021. Data on the heat map represent log2 of sequencing reads assigned to genes. Statistical significance was determined by unpaired, two-tailed Student’s t test in b . * P < 0.05, ** P < 0.01, *** P < 0.001. In each panel, n indicates the number of independent experiments performed.
Wnt Targeted Gene Microarray Expression Profiling System, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wnt-targeted gene microarray expression profiling system/product/Qiagen
Average 90 stars, based on 1 article reviews
wnt-targeted gene microarray expression profiling system - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
wnt target gene  (Basler)
90
Basler wnt target gene
a Representative western blots of the indicated proteins in the MAPK inhibitor resistant human melanoma cell culture M12, at different timepoints of CHIR99021 (6 μM) treatment (timepoints are shown directly in the figure). GAPDH was used as loading control ( n = 3). b RNA levels in MAPK inhibitor sensitive (M98) human melanoma cell culture (ΔCt to GAPDH expression) after 4, 8, and 12 h of CHIR99021 (6 µM) treatment are shown compared with DMSO (ctrl) ( n = 3). c Representative western blot of the indicated proteins in the MAPK inhibitor sensitive human melanoma cell culture (M98). A representative western blot for the indicated proteins of M98 human melanoma cell culture stably expressing shSOX10 or shMITF constructs cultured in presence or absence of CHIR99021 for 24 h, 6 μM. Nssh represents the scrambled shRNA used as control construct ( n = 3). d Representative western blot of the indicated proteins in presence of CHIR99021 (6 μM) and/or MG-132 (20 μM) (in single or double treatments) for 16 h. GAPDH was used as a loading control ( n = 3). e Heatmap demonstrating the gene expression <t>of</t> <t>SOX10</t> and <t>Wnt</t> target genes of M98 and M00 (MAPK inhibitor sensitive cell cultures) and M11 (resistant to MAPK inhibitor) in either presence or absence of CHIR99021. Data on the heat map represent log2 of sequencing reads assigned to genes. Statistical significance was determined by unpaired, two-tailed Student’s t test in b . * P < 0.05, ** P < 0.01, *** P < 0.001. In each panel, n indicates the number of independent experiments performed.
Wnt Target Gene, supplied by Basler, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wnt target gene/product/Basler
Average 90 stars, based on 1 article reviews
wnt target gene - by Bioz Stars, 2026-04
90/100 stars
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Image Search Results


a Representative western blots of the indicated proteins in the MAPK inhibitor resistant human melanoma cell culture M12, at different timepoints of CHIR99021 (6 μM) treatment (timepoints are shown directly in the figure). GAPDH was used as loading control ( n = 3). b RNA levels in MAPK inhibitor sensitive (M98) human melanoma cell culture (ΔCt to GAPDH expression) after 4, 8, and 12 h of CHIR99021 (6 µM) treatment are shown compared with DMSO (ctrl) ( n = 3). c Representative western blot of the indicated proteins in the MAPK inhibitor sensitive human melanoma cell culture (M98). A representative western blot for the indicated proteins of M98 human melanoma cell culture stably expressing shSOX10 or shMITF constructs cultured in presence or absence of CHIR99021 for 24 h, 6 μM. Nssh represents the scrambled shRNA used as control construct ( n = 3). d Representative western blot of the indicated proteins in presence of CHIR99021 (6 μM) and/or MG-132 (20 μM) (in single or double treatments) for 16 h. GAPDH was used as a loading control ( n = 3). e Heatmap demonstrating the gene expression of SOX10 and Wnt target genes of M98 and M00 (MAPK inhibitor sensitive cell cultures) and M11 (resistant to MAPK inhibitor) in either presence or absence of CHIR99021. Data on the heat map represent log2 of sequencing reads assigned to genes. Statistical significance was determined by unpaired, two-tailed Student’s t test in b . * P < 0.05, ** P < 0.01, *** P < 0.001. In each panel, n indicates the number of independent experiments performed.

Journal: Oncogene

Article Title: Temporal activation of WNT/β-catenin signaling is sufficient to inhibit SOX10 expression and block melanoma growth

doi: 10.1038/s41388-020-1267-7

Figure Lengend Snippet: a Representative western blots of the indicated proteins in the MAPK inhibitor resistant human melanoma cell culture M12, at different timepoints of CHIR99021 (6 μM) treatment (timepoints are shown directly in the figure). GAPDH was used as loading control ( n = 3). b RNA levels in MAPK inhibitor sensitive (M98) human melanoma cell culture (ΔCt to GAPDH expression) after 4, 8, and 12 h of CHIR99021 (6 µM) treatment are shown compared with DMSO (ctrl) ( n = 3). c Representative western blot of the indicated proteins in the MAPK inhibitor sensitive human melanoma cell culture (M98). A representative western blot for the indicated proteins of M98 human melanoma cell culture stably expressing shSOX10 or shMITF constructs cultured in presence or absence of CHIR99021 for 24 h, 6 μM. Nssh represents the scrambled shRNA used as control construct ( n = 3). d Representative western blot of the indicated proteins in presence of CHIR99021 (6 μM) and/or MG-132 (20 μM) (in single or double treatments) for 16 h. GAPDH was used as a loading control ( n = 3). e Heatmap demonstrating the gene expression of SOX10 and Wnt target genes of M98 and M00 (MAPK inhibitor sensitive cell cultures) and M11 (resistant to MAPK inhibitor) in either presence or absence of CHIR99021. Data on the heat map represent log2 of sequencing reads assigned to genes. Statistical significance was determined by unpaired, two-tailed Student’s t test in b . * P < 0.05, ** P < 0.01, *** P < 0.001. In each panel, n indicates the number of independent experiments performed.

Article Snippet: Annotated WNT and SOX10 transcriptional target gene lists were obtained from Metacore ( https://portal.genego.com/ ).

Techniques: Western Blot, Cell Culture, Control, Expressing, Stable Transfection, Construct, shRNA, Gene Expression, Sequencing, Two Tailed Test

CHIR99021 is a small chemical compound that competes with ATP for the binding site in GSK3α/β kinase and thereby inhibits GSK3α/β, leading to the activation of WNT signaling. SOX10 protein levels are reduced following pharmacologic, acute β-catenin stabilization, and this is mediated via proteasomal degradation.

Journal: Oncogene

Article Title: Temporal activation of WNT/β-catenin signaling is sufficient to inhibit SOX10 expression and block melanoma growth

doi: 10.1038/s41388-020-1267-7

Figure Lengend Snippet: CHIR99021 is a small chemical compound that competes with ATP for the binding site in GSK3α/β kinase and thereby inhibits GSK3α/β, leading to the activation of WNT signaling. SOX10 protein levels are reduced following pharmacologic, acute β-catenin stabilization, and this is mediated via proteasomal degradation.

Article Snippet: Annotated WNT and SOX10 transcriptional target gene lists were obtained from Metacore ( https://portal.genego.com/ ).

Techniques: Binding Assay, Activation Assay